After a 2-week acclimatization phase, 50 and 150 μM CdCl2 were added to the nutrient medium.
When a ripe female was transferred to the experimental set-up a 1 h acclimatization phase started with the opaque partitions placed between the males and the female.
The seed acclimatization phase was considered to be over when a steady-state condition was observed in terms of equilibrium COD and NH4+-N reduction with respect to a steady level of MLVSS concentration and SVI in the reactor.
It must also be pointed out that the experimental setup allows for settling of suspended solids during the acclimatization phase.
In each trial, females were observed during the acclimatization phase to ensure that they indeed swam along each male's compartment.
Each trial consisted of an acclimatization and a sequential access phase (Egger et al., 2008).
"There's been an acclimatization to corruption.
Following an acclimatization period of three weeks, three experimental diets were offered, each for one group.
In the column experiment, an acclimatization of 23 days took place before steady-state diffusive vapor transport through the horizontal column was achieved.
