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"The solar screens are an absolute expression of what I believe in," she beams.
"People come in their everyday dress," said Clay Sheff, the studio's founder, their looks "an absolute expression of their musical styles".
Hence, memory performance did not reflect the activity pattern of brain regions that represented the unscaled difference between predicted and actual reward or those reflecting an absolute expression of reward magnitude (Supporting Information Table S3).
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It should be noted that this procedure is only semiquantitative and does not reflect an absolute expression level of the test gene mRNA.
Promoters that demonstrate a two-fold increase in expression (post-treatment/pre-treatment expression >= 2) as well as an absolute expression increase of at least 5,000 units are labeled as up-regulated.
The fluorescence intensity obtained from images of cells incubated with QD-FcR-aCLDN4 conjugates can be converted to an absolute expression level of number per square micron on the cell surface using the calibration curve.
Two thousand thirty five genes were expressed differentially between the two cell types when the expression differential had a p < 0.001 (Welch's t-test), a fraction of at least 0.5 and an absolute expression level difference of ≥ 2-fold (Additional file 2, Table S2).
Under basal, chow-fed conditions, our filtering protocol identified 5,993 significantly expressed genes in the small intestine (i.e. having an absolute expression signal >20), of which 253 were transporters and phase I/II metabolism genes.
To determine if the higher proportional expression of phospho-ezrin in the thymus translated into an increased absolute expression of ezrin, the same tissues were analyzed by western blot using antibodies specific for ezrin and normalizing to the housekeeping gene GAPDH.
Therefore, the absolute expression of a single gene in a single sample is not directly comparable between different gene expression platforms.
It would therefore appear that two, largely independent, age effects exist, one connected with the absolute expression of a gene in relation to age (age-factor 1) and the other with the residual variation possibly connected with variation between individuals or with the interrelation of expression of the different genes (age-factor 2).
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