Meanwhile, 9 mutants (R18A, L143A, E153A, G182A/F183A, C374A, C376A/G377A, Y393A, V566A and V571A) produced absolute blue colonies (Fig. 2C), and the recombination frequency was zero as compared with wild-type protein (Table 2).
PCR amplification was run with 3.5 μL of cDNA template in 10 μL of reaction mixture containing 5 μL absolute blue qPCR SYBR green ROX mix (Thermo Scientific) and 300 nM primers.
The qPCR reaction (total volume of 20 μl) was prepared by mixing 10 μl of the 2x concentrated absolute blue solution with 1 μM of each kDNA minicircle specific primers JW11 and JW12 (Table 1) and template DNA (2 μl) [ 12].
I came for a walk and found an absolute true-blue Yorkshireman wandering around in the woods.' It turned out to be Steve Warrillow, the warden who is paid by the factory to manage the reserve on a full-time basis.
"I woke up the morning after the election and saw that Brighton and Hove was now just a tiny red and green island in an absolute sea of blue.
Absolute blue magnitude and absolute infrared magnitude are correlated for all Hubble types.
First, his show on Friday was an absolute pleasure: Balzac and the blues, with a pastoral bride in drawn-up skirts and the village lad.
"It has been an absolute privilege to have led the Blues since its inception," Norster said in October.
But now it's back, bigger than ever, and with an absolute wonder to wash away the blues of the rainy week ahead.
"But we're not a normal business and we have an absolute clear understanding that the Sky Blues should play in Coventry.
Coomassie blue was used as an absolute control for total protein loading.
