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Residue substitutions affected the amplitude of binding, although this was position-dependent (Table 1 and Figure 2).
The amplitude of binding was highest at mildly acidic pH (pH 6.8), declining by 15% at pH 7.2 and a further 20% up to pH 8.2 (Supplementary Figure S2).
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With AFB5 the order and relative amplitudes of binding were similar to the pattern for TIR1, but all dissociation rates from AFB5 were much more rapid.
In addition, density-dependant studies indicated that the amount of ligand immobilized on the surface affected the amplitude of protein binding, but not the affinity, for Akt-PH.
The results obtained with the newly developed force field parameters for the coordination environment of the active-site ferrous ion show that FIH1 undergoes a significant conformational stabilization with a decrease in motional amplitude upon binding of the substrate or the inhibitors.
We investigated if our model would have a higher predictive power when classifying the complexes according to their amplitude of conformational change upon binding.
In a same way, in presence of p75NTR, cyclotraxin-B, like BDNF, gains in binding affinity, amplitude of inhibition and kinetics of action.
Thus the amplitude of methylation gain upon removal of TF binding sites is related to the CG density of the fragment, resembling the prediction from the CG only model.
Nature of binding: None.
The largest group consists of binding functions, specifically nucleotide binding, protein binding, and sugar binding.
Choose the type of binding.
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