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The amplifying procedure of the recombinant H11 baculovirus was performed twice and finally the high-quality, high- titer (2.87 × 108 pfu/ml) master virus stock was obtained.
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Would the immobot incorrectly invoke an emergency procedure, amplifying a minor problem into a major one?
The ΔSPI-3::FRT mutant (ΔSPI-3) was constructed with the same procedure, amplifying the KanR cassette (kanamycin resistance, codified in the pKD4 plasmid) using the primers SPW1 (5'-AACGCAGGCGCTACGTTTGTCGATGCCGTAACTTTCTGAATGTAGGCTGGAGCTGCTTCG-3') and SPW2 (5'-GCTAAATATAGCACGTACTTATTCTTCCAGAAAAAATGGACATATGAATATCCTCCTTA-3').
The procedure for amplifying genomes of laser micro-dissected single cells from frozen tissue sections is outlined in Figure 1A.
Borrelia prevalence was estimated by a standard nested PCR procedure that involves amplifying a portion of the flagellin B (flaB) gene using DNA extractions of individual ticks (Johnson et al. 1992; Clark 2004).
This procedure is designed for amplifying anonymous flanking genomic DNA regions.
High-molecular weight genomic DNA was obtained from a marine environmental sample (Hawaii Ocean Time Series, cruise 186, 75 m depth, [10]) and from laboratory cultures using standard procedures [14], or by amplifying genomes from individually flow-sorted cells as described [15].
A quantification procedure is proposed and involves amplifying a sample with both primer sets and then normalizing the total DNA using the total non-normalized bovine and buffalo DNA.
These amplifiers are confined to amplifying predetermined frequencies.
The other LO signal is acquired through the following procedure: mixing S1 and S2, ×12 frequency multiplication, amplifying, and filtering.
The long-range PCR procedure enabled us to reduce the likelihood of amplifying possible nuclear mitochondrial pseudogenes (numts) [27], [42], [43], [44].
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