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The development certainly seems to have blindsided the international press — Zimbabwean media reported that he had received the award on Sunday to little notice, but as word of the irony spread on social media, Western news outlets began amplifying coverage of the dubious distinction.
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Of the MDA methods, Repli-G generated 3 to 4-fold more amplified DNA, but introduced marginally more bias than GenomiPhi, and generated significantly lower genome coverage when amplifying Halobacterium species NRC-1.
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Still, she said, even allowing for the amplifying effect of the news coverage, something has changed.
The primers amplifying COI-2 showed the highest taxonomic coverage in Actinopterigii and Sauropsida, while those amplifying 16Sr/16Sr2 showed the highest coverage of Amphibians and Mammals.
These amplifiers are confined to amplifying predetermined frequencies.
Prior to using the degenerate primers to identify the female pheromone receptors, we tested and analyzed the coverage of these degenerate primers in amplifying the VR genes.
The purpose for amplifying the three different populations of cDNA fragments was to ensure more even sequence coverage along transcripts by reducing coverage bias towards the 3′termini of transcripts caused by the transcription of truncated mRNA during first-strand cDNA synthesis.
The broad V gene family coverage of our primer set was experimentally validated by amplifying the rearranged/expressed VH and VL genes from splenocytes and a panel of 38 hybridomas under conditions where primer mixes and genomic DNA or total RNA was used as starting template.
The primers were specifically designed to prevent a good match with the pseudogene alleles, so that the coverage of non-functional sequences could be limited while amplifying the rest of the alleles.
The possibility of amplifying eukaryotic and organelle rRNA genes was evaluated by calculating the coverage for each primer candidate and the eukaryotic and organelle rRNA genes that were obtained from the comparative RNA Web (CRW).
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