To experimentally test their validity, we amplified twelve of these introns in a panel of six mammalian species.
In contrast, in a study of small amounts of RNA derived from breast cancer tissue from mastectomy specimens, consistent scaling factor values across all amplified samples confirmed the validity and comparability of the expression data [ 56].
It is reasonable to conclude from these observations that probeset to gene 3' end distance affects the validity of linear amplified expression values.
If Facebook's intention is to encourage lesser-heard viewpoints by subsidizing their rates, the rates should reflect a judgment of that scarcity and the validity of the message it intends to amplify.
The micro-costing approach may have further amplified this lack of generalizability but contributed to the validity of cost estimates.
The elongation factor 2 (EF2) gene of L. striatellus was amplified as an endogenous loading control for testing the validity of template preparation.
When the validity of a predicted gene was in doubt, we amplified a portion of the gene in question from cDNA (carried out for NOS1, NOS2, Pelle, PPO, GNBP3, GNBP11, TEP1).
The validity of these primers, listed in Table 1, was tested using the program Amplify 3.1 for Macintosh.
To test the validity of the transcript sequence prediction of Adamts16 by the NCBI website, we sought to amplify the expressed rat transcript of Adamts16 using several sets of primers encompassing each of the transcript sequence predictions (Fig. 6).
The validity of this result can be pointed out by the fact that RNA was not amplified before the quantification, and that the exact amount of RNA was preserved in each cell type.
The resulting bisulfite converted DNA was amplified in a duplex PCR comprised of oligonucleotides for an internal control assay, actin β for determining the validity of the result and oligonucleotides for the target analyte, methylated Septin9.
