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We'll accomodate both of course, we want to both amplify using existing platforms and at the same time make it as lazy as possible for our members to use Ning.
Sequences containing microsatellites were detected using Tandem Repeats Finder version 4.09 (Benson 1999), and primer pairs were designed for microsatellite loci with suitable flanking regions to amplify using FastPCR software version 6.5.94 (Kalendar et al. 2009).
As a result, ITS2 sequences are relatively easy to amplify using one pair of universal primers selected by our group (Table S1).
However, it is possible that EHDV strains will be identified in the future that cross-react by RT-PCR assay with heterologous serotypes, or fail to amplify using these type-specific primers.
Fourteen had genomic prints identical to that of the clade 2B reference virus, A/South Dakota/06/2007, with two viruses each having one SNP in the PB1 amplicon, one with a SNP in the NP gene target, and one that failed to amplify using the PB1 primers (Figure 5).
As expected, the primer pairs did not amplify using genomic material of BRSV as template.
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Signals were amplified using Multiclamp 700B amplifiers (Molecular Devices).
Signals were amplified using an EPC10-2 amplifier (HEKA Elektronik, Lambrecht, Germany).
The resulting DNAs obtained are amplified using linker-dependent PCR.
The complete cytb was amplified using Thermocycler GeneAmp 9700, USA.
Asx exons were amplified using genomic PCR.
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