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Coastal waters are rich in hydrogenase sequences, as suggested by our analysis of complete genomes (Table 2, 4, Table S1, supporting information), and the number of sequences we could amplify of a single class of NiFe-hydrogenases from the North Sea and the Baltic Sea (Fig. 5).
Additional file 4: Primer sets to amplify of target transcripts.
Seven primers were used to amplify DNA encoding κ light chains, and nine primers were used to amplify of DNA encoding Fd fragments of the γ chain [ 30].
The antibody of Egr-1 was used for immunoprecipitation and the primer set (5′-CAG AGG TCA CTT AAG TAC CCA GCC ATC T-3′ and 5′-ATA AGC TTT GCA GTC TGC CTG AGC ATC C-3′) were used for amplify of mouse PlGF promoter according to the manufacturer's instructions.
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Table S2: List of the primers used to amplify segments of the BADH2 gene.
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Republican congressional leadership has pledged to amplify all of these conversations if it gains control.
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