The Smart-EP version 10, modified for high frequency capability and coupled to high frequency transducers was used to generate specific acoustic stimuli, and to amplify, measure, and display the evoked brainstem responses of anesthetized mice.
Although Labour currently presents a radical offer on climate change, such a pact would amplify the measures needed to stave off what is, after all, an existential threat to humanity.
The proposed signal amplification strategy is an integrated "biological circuit" designed to initiate a cascade of enzymatic reactions in order to detect, amplify, and measure a specific miRNA sequence by using the isothermal cleavage property of a DSN.
"Each time the particle passes one electrode... it induces a current to pass through a resistor, and that current we amplify and measure," Khabbaz explains.
The primers and conditions used to amplify and measure mRNA are listed in Supporting Information S6A.
Integrated DNA Technologies supplied probes, and we used a Bio-Rad (Hercules, CA) IQ-Cycler real-time PCR system to amplify and measure fluorescence of aromatase and 18S.
This cDNA was amplified and measured by using TaqMan® Gene expression assays (Applied Biosystems).
Two endogenous controls (GAPDH and β-Actin) were amplified and measured in separate wells as real-time reporters.
After adding AttoPhos (Promega), the signal was amplified and measured with a Victor2 microtiter plate reader (PerkinElmer, Boston, MA).
After completion, the portion of solution that was not amplified was measured on the FET with either the RE or the QRE, followed immediately by the amplified solution.
The early cross-departmental meetings served as brainstorming forums to amplify cross-departmental measures that could better connect undergraduates and other faculty with interactions between biology and mathematics.
