Sentence examples for amplify a known from inspiring English sources

The assay uses RT-PCR to amplify a known H5N1-specific region of 768 nucleotides.

To further test the accuracy of our assay in comparison to its TaqMan counterpart, we used our BRAF tail assay to amplify a known template mixture (80% wild-type template and 20% mutant template).

Each library was tested for enrichment of methylated DNA by performing PCR using a primer pair designed to amplify a known methylated gene (LIT1) as a positive control as well as a primer pair that amplifies an unmethylated gene (MP68) to ensure adequate depletion of unmethylated DNA.

To measure TRECs in samples we have used a quantitative real-time PCR (TAQMAN™) assay to amplify a known proportion of the extracted DNA.

Following elution of bound complexes, ethanol precipitation, and resuspension of MeDIP DNA, a small aliquot of DNA and control input DNA were used to amplify a known methylated DNA region and a known unmethylated DNA region by real-time PCR.

Their results confirm and amplify a well known problem; the specificity of current methods is still too low.

Primers were designed within the reverse transcriptase domain using Primer3Plus [ 45] to amplify all known elements from a family.

Additionally, the neuraminidase gene was amplified, using primers designed to amplify all known neuraminidase subtypes of influenza A virus [ 13], sequenced, and found to be of the N8 subtype.

If the receptor and its ligand are in close proximity of interaction, the oligonucleotide would direct the formation of a circular DNA molecule before being amplified, a process known as rolling circle amplification.

We report a novel method for molecular subtyping of avian influenza A virus hemagglutinin genes using degenerate primers designed to amplify all known hemagglutinin subtypes.

Therefore, we screened the clones by RT-PCR utilizing a panel of 24 different Vα specific primers that we designed to amplify all known mouse Vα gene segments.