USFB1567 and ELR are the primers used to amplify the coding regions of all mitochondrial minichromosomes.
Primers were designed to amplify the coding region corresponding to the mature peptide and its exact sequence was confirmed.
The procedure has been employed to successfully amplify the coding sequence of 10 human cancer genes in one assay with uniform abundance of the final products.
A possible explanation is that the primer pair USFB1567 ELR, which we used to amplify the coding regions of the mt minichromosomes of the elephant louse, are not conserved in the minichromosome(s) that contain trnD, trnN, trnS1 tct) and nad2 genes; these minichromosome(s) were thus not amplified by the PCR with USFB1567 ELR.
We designed a multiplex, polymerase chain reaction based primer panel to amplify the coding regions of the 28 ALS-related genes and sequenced DNA samples from 257 Japanese ALS patients using an Ion Torrent PGM sequencer.
To amplify the coding sequence of CsLEA11, the primers 5′-aaaaGGATCCATGGCGAATGTACGCGATGAG-3′ (BamH I restriction site underlined) and 5′-aaaaAAGCTTATGATGGTGGCCAGGTAATTTC-3′ (Hind III restriction site underlined) were designed.
These primers were designed to amplify the coding region of all VEGF isoforms and to contain restriction endonuclease sites (BamHI and XhoI) for sub-cloning into pET21a vectors.
In order to investigate the functions of OsGLRs, we amplified the coding sequences (CDS) of OsGLR1.1, OsGLR1.3, OsGLR2.1, OsGLR2.3, OsGLR3.2, OsGLR3.3 and OsGLR3.4, which have higher expression levels according to the previous qRT-PCR results.
Primers GWBlb1F (5'-CACCATGTTGTAATTATTGGCGAAC) and ARO518 (5'-GTTGTTATAAGGGTATAAGTGAGC) were designed to amplify the coding region of Rpi-blb1.
We used the Oligo 6.2 program (NBI) to design specific primers to amplify the coding exons and the adjacent intronic sequences of the ACE gene (26 exons).
To minimize the number of PCR reactions required to amplify the coding and near coding genomic sequence of SLC26A5, a combination of standard and long PCR was used.
