Sentence examples for amplified with time from inspiring English sources
We wanted to find out why people would ever willingly throw themselves into a situation where the intensity and chaos of dating is amplified with time limits and forced interactions.
These differences were amplified with time, so that after 7d, bone marrow cells subjected to mechanical stimulation had differentiated into osteoblasts (n = 8; Fig. 2C).
As shown in Figure 3B, dsRNA of about 50 bp was amplified with time on a non-denaturing PAGE.
As shown in Figure 2B, when NV3Dpol and the RNA template were incubated at 30°C and analyzed on a denaturing PAGE, two reaction products of 50 nts and 90 100 nts were amplified with time.
Then, coprecipitated and WCE DNAs were amplified with real-time qPCR.
To analyse the exonic SNPs c.729C → T and c.975C → G, 10 ng of genomic DNA was amplified with real-time PCR monitoring of fluorescence signals with an ABI Prism 7700 Sequence Detection System (Applied Biosystems).
All but one sample yielded a clear result (figure 3b) at the first amplification (this sample was subsequently successfully amplified), with the total time required for sex identification (processing, PCR, restriction of product and analytical gel) being less than 8 hours.
The EEG was amplified with a 0.3-s time constant and a 120-Hz low-pass filter, and sampled at 500 Hz.
The reaction was amplified with iCycler iQ MulticolorReal Time PCR Detector (Bio-Rad) for 37 cycles with meltingat 94 °C for 30 s, an annealing at 58 °C for 30 s, and extension at 72 °C for 1 min in iCycler iQ PCR 96-wellplates (Bio-Rad).
DNA was amplified with the 7500 Real Time PCR system (Applied Biosystems) by using the following temperature program: 50°C for 2 min, 95°C for 10 min, followed by 50 cycles of 95°C for 15 s and 60°C for 1 min. Amplification data were analyzed by instrument software (Applied Biosystems).
Each gene was amplified with the specific real-time PCR primer set, and was normalized to the control (rp49 for Drosophila and actin for C. elegans).
