These changes may reflect the impact of changes in dietary composition and/or nutrient intake as well as an effect of sibutramine-amplified weight loss in improving insulin sensitivity in the insulin-resistant T2D patients.
The primers L-CYTBF and H-CYTBF were designed in the mitochondrial cytochrome b (cytb) gene and the molecular weight of amplified fragment was 348 bp and amplified the fragment from processed billfish meats.
SSR: simple sequence repeats; QTL: quantitative trait loci; CAPS: cleaved amplified polymorphic sequences; CW: whole cocoon weight; CSW: cocoon shell weight; CSR: cocoon shell ratio; PW: pupal weight.
The amplified products were compared with molecular weight standards (DNA Molecular Weight, Marker 100, Sigma-Aldrich, St . Louis MO, USA).
However, we also detected differences in mean molecular weight for the amplified fragments (allele size range) produced by different groups.
This is due to the relatively unbiased amplification by φ29 DNA polymerase and the high molecular weight of the amplified DNA compared with PCR-based methods.
The amplified products along with Molecular Weight Marker VI (Roche) were size fragmented on 1.5% agarose gel at 100 V. DNA fragments were stained with ethidiumbromide and visualized under UV light.
The reason for this can be explained by the conservation of flanking regions and repeat sequences in majority of the Arachis species, but seven out of the twelve CS EST-SSR primer pairs amplified products of higher molecular weight.
Plasmid DNA cloned with HBV or HCV sequence was added to a LAMP reaction solution containing both HBV primers and the probe (FITC-labeled) and HCV primers and the probe (ROX-labeled) and amplified, after which low molecular weight PEI (Mw = 600; 0.2 μmol as a monomer) was added.
Molecular weights of the amplified bands were determined based on the relative migration of standard 100 bp DNA ladder (Thermo scientific, USA) in the gel.
Amplified product bands were compared with molecular weight standards (DNA Molecular Weight, Marker 100, Sigma-Aldrich, St . Louis MO).
