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We amplified total RNA from tumor BC2, either with or without the cleanup step of Sephadex™ G-50.
The yield of amplified total DNA was about 300 ng per microliter.
This can be easily detected by PCR amplification as the ITS sequences of the amplified total rDNA population present.
Northern blot analysis with amplified total cRNA confirmed the increase in mRNA levels of several selected genes.
The total dose of amplified alleles ranges from six to eight (Table 4), therefore, the total dose of null alleles ranges from two ('Korona') to zero.
Yields of amplified gDNA were typically in the range of 8 10 μl/50 μl WGA reactions.
All tested genes were found in all FFPE and Bst amplified samples, and their relative gene copy number was within 3-fold range of non-amplified samples.
Estimates of total losses range from $30 billion to $60 billion.
GC content of the amplified products ranged from 35% to 58%, with an average of 43%.
Amplified fragments (ranging from 200 to 600 bp) of the ncRNA31 locus of the A1501 genome are shown in Additional file 1: Table S2.
PCR amplification (30 cycles) was performed in a total volume of 25 µl and the amount of each cDNA was adjusted for each primer pair to be in the linear range of amplification and to give the same quantity of amplified complementary DNA as with the EEF1α primers.
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