Formalin-fixed, paraffin-embedded melanocytic lesions, previously classified according to their tumour growth phase (RGP, VGP, metastasis), were screened for TSPAN8 protein expression by a biotin streptavidin-amplified technique with an alkaline phosphatase kit (Dako-LSAbiotin streptavidin-amplifiedmany).
In this apparatus the light scattered by a laser of 532 nm wavelength, is detected at 173° with respect to the incoming ray (Back Scattering technique), amplified and then analyzed by a correlator.
Few molecular tools are available to investigate epigenetic variation in non-model species, but the Methyl-Sensitive Amplified Polymorphism (MSAP) technique provides data on cytosine methylation without a priori knowledge of genome sequences [22].
Isolates were typed by the random amplified polymorphic DNA technique (RAPD) using two primers: 1247 and 1290 [ 20] (Table 2).
This promoted us to establish a dCAPS (derived Cleaved Amplified Polymorphic Sequences) technique as a detection method.
In that group, all 50 ESBL and 50 ESBL isolates were recognised in typing by the randomly amplified polymorphic DNA technique as separated clones.
PCT plasma concentrations were measured using an automated sandwich immunoassay based on the TRACE (time-resolved amplified cryptate emission) technique, in accordance with the manufacturer's protocol (Kryptor; Brahms Diagnostica, Berlin-Henningsdorf, Germany).
We applied an arbitrarily primed PCR-based technique, Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR), to investigate DNA methylation profiles in three different cell types obtained from Holstein bulls.
The specificity and negative predictive value of PCT have been substantially enhanced in newer assays by the improved functional assay sensitivity of an amplified cryptate emission technique [ 6, 7].
CAPS (Cleaved Amplified Polymorphic Sequences) and dCAPS (derived Cleaved Amplified Polymorphic Sequences) techniques have been used to convert EST based polymorphisms into SNP markers [ 18].
A monoclonal antibody to human estrogen receptor protein (H222 Sp gamma), amplified via immunoperoxidase techniques, was used in the analysis of estrogen receptor in 452 breast carcinomas, 100 endometrial carcinomas, and 15 melanomas.
