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Several, including a pencil sketch of part of Mr. Reich's seminal "Drumming" (1970) and a handwritten description of Mr. Glass's "1+1 for One Player and Amplified Table Top" (1968), on lined legal paper, hang near the Cage.
The individual truncated fragments pgkFL1 to pgkFL5, were PCR amplified (Table 1) and the resultant DNA fragments were inserted in pET28a vector (Novagen).
PDGFRA copy number changes (ratio >2) were observed in 52.6% (30 out of 57) of glioma patients: 18 displayed ratios <5 and were considered representative of aneuploidy/aneusomy and 12 (21.1%) harboured ratios ⩾5 and were considered amplified (Table 1).
Either partial, or complete ORFs for cloning into the E. coli expression system were amplified (Table 1).
Five percent of the seminal plasma samples from heterosexual men contained HIV-1, but XMRV nucleic acid was never amplified (Table 1).
DNA extracts were prepared and, using the polymerase chain reaction (PCR), both multi-copy and single copy target loci were amplified (Table 1) and sequenced to confirm their identity.
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Of the 47 successfully amplified loci (Table 1), 26 were polymorphic (Table 2).
In S. dioica x S. latifolia (dam x sire) crosses all male F1s (N = 9) amplified AP3Y and not SlAP3A, whereas all female F1s (N = 12) amplified SlAP3A (Table 1).Reciprocal crosses between the species showed a similar pattern: all male F1's (N = 15) amplified AP3Yand only one of fifteen males amplified a product determined to be SdAP3A.
The 31 cross-amplifying primer pairs were further tested in P. armata, of which 29 amplified successfully (Table 1).
Kolmogorov distances also indicated that differences in the distribution of DNA copy number existed between the unamplified and amplified samples (Table 2), and that these differences were greater than the differences between the REPLI-g and GenomiPhi amplified samples (Table 7).
Interestingly, younger patients were less likely to have CCNE1 amplified tumors (Table 1 and Figure 6A) suggesting a difference between the etiology of these tumors.
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