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After incubation with the primary antibody, the sections were incubated with a horseradish peroxidase (HRP -conjugated biotin–streptavidin amplified system (Dako) and tHRP -conjugated visualised with 3,3′-diaminobiotin streptavidino) amplifiedbed previousystemayyazi et al, 2000).
The low repetition rate of the amplified system (250 kHz) also provides more dead time between pulses (1 pulse every 4 µs compared to 1 pulse every 12.5 ns for a 80 MHz Ti Sapph) to let the photochemical system return to its fundamental state before the next pulse, hence reducing the photo- and thermal damage [ 22].
After incubation with the primary antibody, the sections were incubated with an AP-conjugated or an HRP-conjugated biotin streptavidin amplified system (Dako) and the signals were visualised with fast red or AEC (Dako) as described elsewhere (Schweyer et al, 2000).
To visualise bound primary anti-IFN γR α, anti-IFN γR β, anti-active caspase-3 and p-STAT-1 antibodies, cells were incubated with AP-conjugated or HRP-conjugated biotin streptavidin amplified system (Dako) as described previously (Schweyer et al, 2000).
Biotin-Avidin system (BAS) is a new biological reacted amplified system.
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The sounds, from amplified systems, or from radios, were those of the Koran.
Polarity change of the resist polymer along with depolymerization and crosslinking strategies has been widely employed in chemically amplified systems.
The continuing growth of sequencing databases with now more than 1700 published bacterial genomes (according to GOLD: http://www.genomesonline.org) suggests that our approach should already be applicable for less amplified systems.
The oscillators operate at a fixed repetition rate around 80 MHz and amplified systems are typically constrained to a few kHz.
With the help of the well-designed and high-quality CHA amplification, the assay could yield 300-fold amplified signal comparing that from non-amplified system.
Levers and linkages: mechanical trade-offs in a power-amplified system.
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