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The triply amplified strategy showed high selectivity, stability, and applicability for detecting the desired protein.
The results demonstrated that the multiple signal amplified strategy we developed was feasible for clinical ATP assay and would provide a promising model for the detection of other small molecules.
In this work, an electrochemical immunosensor, basing on a dual signal amplified strategy by employing a biocompatible polypyrrole film-Au nanocluster matrix as a sensor platform and multi-enzyme-antibody functionalized gold nanorod as an electrochemical detection label, is established for sensitive detection of ofloxacin (OFL).
b The linear relationship between ΔI and OTA concentrations Table 1 Comparison with other reported aptasensors for OTA detection Amplified strategy Detection limit (pg/mL) Linear range (ng/mL) References Enzyme-labeled 1 0.005–10 [27] AuNPs-GO 0.3 1 × 10−3 50 [28] AuNPs-GO 30 0.1–200 [29] QDs 0.64 – [30] CAs 0.10 0.10–10 This work.
In this work, a new signal amplified strategy was constructed based on isothermal exponential amplification reaction (EXPAR) and hybridization chain reaction (HCR) generating the hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-mimicking DNAzyme) nanowires as signal output component for the sensitive detection of thrombin (TB).
Our platform employs an AuNP-based, exonuclease III (Exo III) amplified strategy to achieve colorimetric SNP detection at low nanomolar target concentrations.
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We describe a novel label-free amplified multifunctional strategy of dendritic electrochemical DNA sensor based on terminal deoxynucleotidyl transferase (TdT).
Here, we report a thionine mediated para-sulfonatocalix[4]arene capped AuNPs (pSC4-AuNPs) multilayers signal amplify strategy, which integrates nanoassembly with hybrid macrocyclic supramolecules nanomaterials for the sensitive detection of acetylcholinesterase (AChE) activity, by taking the assay of a neurological diseases marker as an example.
Specific products were preferentially amplified using this strategy, which were verified using DNA sequencing.
Abundant templates can be amplified using a strategy that uses 6 overlapping amplicons to cover the complete viral genome, whereas scarce templates can be amplified using a strategy that uses 11 overlapping amplicons of smaller size.
The PCR strategy amplified a region that ranged from 1,084 nt (CVB1) to 1,174 nt (CVA6).
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