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Figure 1 Satellite system architecture supporting K carriers amplified simultaneously by a single onboard HPA.
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An alternative approach is to use multi-targeted PCR, in which several target genes are amplified simultaneously.
For standardization and quantification, rat 18S was amplified simultaneously.
Mushroom DNA was extracted and then amplified simultaneously to test for contamination during DNA extraction.
Briefly, using bisulphate treated genomic DNA, we amplified simultaneously 12 BAGE loci in a single PCR.
GAPDH was amplified simultaneously.
Consequently, the two transcripts were amplified simultaneously.
Input samples were amplified simultaneously as the internal controls.
Briefly, the DNA template was amplified simultaneously with two forward and two reverse primers, and the PCR products were diluted, added to a formamide-ROX mixture and subsequently resolved by capillary electrophoresis (3730xl DNA Analyzer; Applied Biosystems).
All cDNA samples of each treatment were amplified simultaneously in one PCR plate.
All samples were amplified simultaneously in triplicate in a one-assay run.
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