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Coupled to a gold nanoparticle-based colorimetric assay, the amplified recognition signal can be quantitatively detected or visually read with the naked eye.
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PCR-amplified fragments that harbor a difference in size: including SSRs; CAPS: Cleaved Amplified Polymorphic Sequence: PCR-amplified fragments that harbor a polymorphism in a restriction enzyme recognition sequence.
Colloidal platinum can be functionalized with nucleic acids and has been used as label for the amplified biorecognition of DNA hybridization, aptamer/protein recognition events and tyrosinase activity [37].
For the initial set of experiments screening for the loss of a PstI restriction site situated between the PB1 recognition sites, the PCR amplified fragments were digested overnight at 37°C with 20 U PstI (New England BioLabs) in 1x NEbufferfer.
The forward primer was designed to introduce a FokI site (an isoschizomer of BstF5I) in the amplified product by substituting the restriction recognition sequence GGATG for the ninth base upstream of the polymorphic nucleotide.
The amplified DNA fragment contained internal XbaI recognition site between the promoter and RBS of Φ10 gene of T7 phage providing translation of lacI gene in the pMW-P lac UV5 -lacI-118 plasmid.
The 3′-UTR of Bmi1 mRNA containing the intact miRNA-218-5p miRNA-218-5p miRNA-218-5pces were PCR-amplifamilynd subcloned into the SacI and SalI sites of pmirecognition.
Restriction fragment length polymorphism analysis of the RT-PCR products indicated nucleotide substitutions in the EcoRI recognition sequence site in the amplified genome region of the isolates.
By digesting the amplified products with 8 restriction enzymes having different recognition sites, a total of 27 polymorphic marker fragments were obtained.
This chemistry has proven useful in the area of biosensing, where the formation of a hydrogel on the time scale of seconds serves as a macroscopic, amplified signal that can be connected to molecular recognition events.
Last night, Michael A. Cardozo, the city's corporation counsel, said in a statement, "We are pleased with the court's decision and its recognition that the city's regulation of amplified sound does not violate individuals' constitutional rights".
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