Sentence examples for amplified quantity of from inspiring English sources

This amount was then added to the sample containing the lowest amplified quantity of reference virus, corresponding to 0.001 TCID50 prior to the extraction step.

The relative quantity of amplified cDNA corresponding to each gene was calculated by using the ΔΔCt method and normalized for expression of gapdh in each sample.

PCR amplification (30 cycles) was performed in a total volume of 25 µl and the amount of each cDNA was adjusted for each primer pair to be in the linear range of amplification and to give the same quantity of amplified complementary DNA as with the EEF1α primers.

We analyzed the quality and quantity of amplified RNA with Bioanalyzer 2100 (Agilent, CA, USA).

The quantity of amplified DNA was determined with a Nanodrop spectrophotometer (Nanodrop Products, Wilmington, DE, USA).

Changing requirements for the type and quantity of amplified RNA, driven by evolving microarray technologies, have led to the development of novel amplification strategies.

We recommend testing a range of dilutions of the primary PCR product to obtain the desired quantity of amplified cDNA in 9-10 Pcyclesles.

Quality and quantity of amplified RNA from both the first and second round of linear amplification, and fragmented RNA, were analyzed on Agilent Bioanalyzer with a nanokit (Agilent Technology).

From all other samples (control and experimental reactor) the quantity of amplified DNA was below the detection limit for visualization using ethidium bromide staining.

After purification, the quantity of amplified cDNA was estimated using an ND-1000 spectrophotometer (NanoDrop) and the quality was evaluated using an Agilent 2100 Bioanalyzer.

For each initial template quantity, we looked at the threshold cycle, the Ct, corresponding to the number of cycles required to reach a set quantity of amplified cDNA during the exponential phase.