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This amount was then added to the sample containing the lowest amplified quantity of reference virus, corresponding to 0.001 TCID50 prior to the extraction step.
A total of 117 arrays were used in this study, each probing equal quantities of amplified mRNA (2 ug).
Only samples that yielded sufficient quantities of amplified RNA (approximately 5 μg) were used for expression profiling.
Only 127 PCR reactions (1.1%) with S. salar DNA yielded low quantities of amplified products, despite optimization.
The comparative ΔΔCT method was used to evaluate the relative quantities of each amplified product in the samples.
The comparative ΔΔCt method was used to evaluate the relative quantities of each amplified product in the samples.
The comparative CT method was used to evaluate the relative quantities of each amplified product in the samples.
In this study, we evaluated two commonly used methods of WGA for their ability to produce large quantities of uniformly amplified DNA with minimal introduced mutations.
The relative quantities of the amplified probes in each sample were determined using Genotyper Applied Biosystemss, Foster City, CA, USA) and Excel (Microsoft Corp., Redmond, WA, USA) software (Gene Marker version 1.5; Softgenetics Inc, State College, PA, USA).
We believe that our approach to generation of abundant quantities of good quality amplified product will find many applications in genomic research.
For this study we mixed and amplified known quantities and proportions of normal leukocyte and serum DNA, as described before [ 18].
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