Sentence examples similar to amplified quantities from inspiring English sources

This amount was then added to the sample containing the lowest amplified quantity of reference virus, corresponding to 0.001 TCID50 prior to the extraction step.

For this study we mixed and amplified known quantities and proportions of normal leukocyte and serum DNA, as described before [ 18].

Amplified RNA (aRNA) quantity was evaluated spectro-photometrically by readings at 260 nm (A260) and 280 nm (A280).

A total of 117 arrays were used in this study, each probing equal quantities of amplified mRNA (2 ug).

In parallel, a universal reference RNA sample was created from the spleen of an outgroup of animals (n = 4), which was further amplified to achieve large quantities using Amino Allyl MessageAmp™ II aRNA Amplification (Ambion).

Only samples that yielded sufficient quantities of amplified RNA (approximately 5  μg) were used for expression profiling.

Only 127 PCR reactions (1.1%) with S. salar DNA yielded low quantities of amplified products, despite optimization.

The relative quantity of amplified cDNA corresponding to each gene was calculated by using the ΔΔCt method and normalized for expression of gapdh in each sample.

PCR amplification (30 cycles) was performed in a total volume of 25 µl and the amount of each cDNA was adjusted for each primer pair to be in the linear range of amplification and to give the same quantity of amplified complementary DNA as with the EEF1α primers.

We analyzed the quality and quantity of amplified RNA with Bioanalyzer 2100 (Agilent, CA, USA).

The quantity of amplified DNA was determined with a Nanodrop spectrophotometer (Nanodrop Products, Wilmington, DE, USA).