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The expanded genetic diversity present in such populations and the amplified number of recombination events mean that QTL can be identified at a higher resolution.
Such a structure leads to an amplified number of recombination events, which means that any QTL can be mapped at a higher resolution.
Ingenuity Upstream Regulator Analysis® identified multiple transcription factors (mainly ligand-activated nuclear factors) controlling a greatly amplified number of lipid metabolism genes in abdominal fat during juvenile development (1 11 wk) of FL and LL chickens (see Figure 9).
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The resulting cDNAs were PCR-amplified (number of cycles indicated in Additional file 14A) using high-fidelity DNA polymerase.
Shear stress-induced up-regulation and increased activity of endothelial nitric oxide synthase are related to increased nitric oxide production by endothelial cells [28], contributing to amplified number and activity of circulating EPCs [3].
NCIH716 has a tumor cell line, a FLT3 amplified copy number of around 3.5 copies per genome.
Since our three cDNA libraries were neither normalized nor amplified, the number of ESTs in a contig should represent the abundance of the corresponding transcript in the libraries.
The amplified band number of mGing was much higher than that of mPing, which made it difficult for the polymorphic bands to be visible in mGing TD assay.
To ensure that the PCR products were not over amplified, the optimal number of PCR cycles was determined according to manufacturer's guidelines.
Abbreviations: bp, length of the amplified sequence; nH, number of haplotypes; Ts, transition; Tv, transversion; ∑, total; PS, polymorphic sites; π, nucleotide diversity; f, frequency; T93, Tamura-Nei genetic distance (1993); L'sP, Lake's paralinear genetic distance.
In three independent experiments performed with amplified DNA the number of aberrations varied from 38 in experiment #1 to 36 in experiment #2 and 35 in experiment #3, compared to a total of 24 micro-CNAs when non-amplified DNA was used for analysis.
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