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This secondary antibody is pre-conjugated with a known DNA sequence that is amplified in the subsequent LAMP reaction after release from the secondary antibody.
After we performed Re-ChIP analysis, immunoprecipitating the NF-YA-containing complexes with an antibody against p300, only those DNA sequences that are simultaneously bound by both proteins would be amplified in the subsequent PCR.
Adding these sequences enables the fragments to be amplified in the subsequent PCR step.
Only probes that are properly ligated can be amplified in the subsequent PCR reaction.
With the second strand partly degraded only the first strand is amplified in the subsequent PCR.
With the second strand degraded, only the first strand is amplified in the subsequent PCR.
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"That signal is amplified in the patient's brain.
This decrease was clearly amplified in the presence of GdBN.
They reported a HER2 discordance rate of 10% with one negative primary lesion becoming amplified in a subsequent lung metastasis; and one HER2 amplified primary lesion being associated with loss of amplification in a liver metastasis.
Virus was either amplified in 2 subsequent steps to generate P1 and P2 virus stock or used to generate Baculo Infected Insect Cells (BIIC) as described previously [ 23].
It was surprising to find that the ACV-resistant virus selected in vivo was not retained or amplified in subsequent passages despite continued suboptimal treatment with VCV.
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