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Kicked up from the seabed, the tsunami amplified in size and slowed in speed as it moved into the shallows beside the Japanese coastline, and by the time it touched land it was a wall of water, black and smooth.
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The cDNA amplification strategy is schematically reported in Additional file 4: the transcript was amplified in seven fragments ranging in size from 1.1 kb to 1.9 kb, with overlapping sequences of at least 188 bp.
Capillary array electrophoresis with these primers revealed products of two sizes amplified in the available parents of the crosses made to produce the F2 backcross families (Table 2).
In summary, the mtDNA of P. mammillata was amplified in three long overlapping fragments (size range: 2.5 – 9.4 kb), whereas the mtDNA of P. fumigata was amplified in five overlapped fragments (size range: 1.2 – 5 kb).
A PCR product of the expected size was amplified in at least one of the Fagaceae or Nothofagaceae species for 91.8% (223/243) of the EST-SSRs tested.
Fragments of the expected size were amplified in all individuals (Fig. 5B) and DNA sequence analysis revealed high sequence similarities to the respective LycEPRV regions.
In this case, amplicons of different sizes are amplified in one or more multiplex mixtures.
Four different amplicon sizes are amplified in parallel in a single tube from cDNA (multiplexed endpoint RT-PCR assay) to minimise the consumption of what are often limited amounts of RNA.
The size effect is amplified in the case of counter-diffusion, and leads to possible enhancement in diffusivity even in counter-diffusion.
However, in most cases, significant amounts of cDNA of the expected size were only amplified in reactions containing RNA of the same topotype.
Instead of subdividing exons 11, 10, 14 and 27 due to their size, each were amplified in one fragment, using primers located in the adjacent introns.
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