Sentence examples for amplified first from inspiring English sources

cDNAs were amplified first from total viral RNA using Superscript Reverse transcriptase (Invitrogen) and the universal primer method described by Hoffmann and al. [31].

The V regions of heavy and light chains, CH1 (with IgG1 isotype) and CL (including κ and λ) were amplified first.

Due to the fact that RNA is amplified, first through RT-PCR, and then by in vitro transcription, milligram-scale RNA masses can be achieved, sufficient to transfect large numbers of autologous DCs.

The cDNAs were amplified first using the primer pair 5'-CCCACAGAAGAGCAGCCCAA-3' and 5'-GACTAGCTGGAATTCGCGGTTAAA-3' and then using the primer pair 5'-TCCTGGACACGCTCCTGCAA-3' and 5'-GACTAGCTGGAATTCGCGGTTAAA-3'.

For ex vivo HIV LTR detection from HIV-infected patient blood, extracted DNA had to be amplified first using a complete whole genome amplification kit (Sigma-Aldrich, UK) due to small cells numbers.

For both species, the partial regions rrnS-rrnL and rrnL- cob were amplified first.

This product was used in PCR as in [ 45] to amplify first several short fragments of cox1, rrnL, and cob using primers found in [ 45- 47].

The piece had a likably propulsive buzz, heartily amplified; the first movement sounded like Bernard Herrmann's "Psycho" shrieks made to jitter and surge à la John Adams's "Shaker Loops".

When we looked at which genes were being amplified, we first noticed that some of these genes are unique to the strain in which the LSE was observed.

The ligated fragments were selectively amplified a first time using MseI primer (5'-GATGAGTCCTGAGTAA-3') and BstYI primers (5'-GACTGCGTAGTGATCCN-3 and 5'-GACTGCGTAGTGATCTN-3').

A full length ptrn-1a cDNA was amplified from first strand cDNA using oligonucleotides 4741 and 4742, digested with SbfI and NheI and inserted into NM2704.