We have previously shown that recombination during PCR can contribute to the complexity of amplified DNA (Meyerhans et al, 1990).
Differential hybridization is usually a result of single-nucleotide polymorphisms (SNP) that affect the presence of restriction sites (and, therefore, certain amplified fragments) in the complexity reduction.
This complexity is further amplified in the presence of multiple conflicting objectives, for which the goal is to generate trade-off compromise solutions, commonly known as Pareto-optimal solutions.
This study, which contributes to building a theoretical model, suggests that anxiety and perceived threats to professional competence can be experienced at multiple levels, and are amplified with regard to the complexity of adolescent presentations.
We have further documented that calibration of the number of PCR cycles used in this method allows amplification without loss of either relative mRNA copy abundance or complexity of the amplified product.
One possibility for why 36% more were found in the M' group is that the complexity of the T7 amplified transcripts is greater.
We initially closed gaps by primer walking via PCR and Sanger sequencing the amplified region, however, due to the complexity of numerous repeat regions, this strategy was very tedious and difficult.
By adding a few (typically <4), selective base pairs (bp) to these primer sequences, the amplified fragment pool is reduced in complexity such that a suitable number of fragments can be visualized [ 3, 4].
Inspection of the over-amplified regions reveals sequences of low complexity and numerous repeat patterns.
After initial tests of five complexity reduction methods through gel electrophoresis of amplified products (data not presented), the ' PstI/ TaqI' method was chosen for library construction.
Although we have relatively good knowledge of which genes can be point-mutated, amplified or deleted in these cancers, the sheer number and complexity of their genome rearrangements has made it difficult to identify genes at chromosome breakpoints [ 2].
