Sentence examples for amplification was done in triplicate from inspiring English sources

Quantitative Real Time PCR amplification was done in triplicate using primers specific for PTK6 exon 2 (PTK6-X2_For 5'-CGGAACCGTG GTTCTTTG-3' and PTK6-X2_Rev 5'-ACTCGGCTTC TCGCTGAC-3') and exon 8 (PTK6-X8_For 5'-TGTTCCTGCT CTTCCCandT-3' and PTK6-X8_Rev 5'-TGGGAGGAAA GAACCCTTGA-3') as described previously [64].

Amplification was done in triplicate.

All Q-PCR amplifications were done in triplicate, and the experiments were performed at least twice.

The bead immobilized PCR product was purified, washed, denatured using a NaOH solution, and washed again using the Pyrosequencing Vacuum Prep Tool (Pyrosequencing, Inc .. PCR amplifications were done in triplicate and the extent of methyl cytosine relative to the total cytosine and thymine at each of 4 CpG sites was measured.

Each amplification reaction was done in triplicate and the specificity of amplicons was confirmed by the presence of a single peak.

This process was done in triplicate to minimize PCR amplification biases.

Amplification of the matK gene from the five fresh plant samples was done in triplicate.

The analysis was done in triplicate.

Each experiment was done in triplicate.

The procedure was done in triplicate.

Each measurement was done in triplicate.