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Results have shown that the optical signal amplification using the nonlinear ring resonator is plausible.
Finally, metagenomic DNA from soil samples was subjected to 16S rRNA gene amplification using the newly designed primers.
Shared DNA fragments containing bar-coded shRNA constructs were subjected to two-round PCR amplification using the primer pairs listed in Supplementary Table S8, according to the instructions provided by Cellecta.
In vitro amplification using the polymerase chain reaction followed by oligonucleotide hybridization can be used to detect murine CMV DNA.
Signal amplification using the Stanford instruments SR560 digitisers ranged up to 5000 fold.
Submaxillary salivary gland biopsy specimens from BALB/c mice latently infected with murine CMV were examined for murine CMV DNA by in vitro enzymatic amplification using the polymerase chain reaction preceding oligonucleotide hybridization.
PCR amplification using the ITS1-F and ITS4 primers yielded a PCR amplicon from each species that differed in size by approximately 200 bp on an agarose gel.
The entire gene for PaXynG was obtained by PCR amplification using the above primers and genomic DNA of P. antarctica GB-4 0) GB-4 0plase.
The PCR products were diluted 1: 29 with sterile double-distilled water prior to the nested amplification using the general primer pair R16F2n/R2 (Gundersen and Lee, 1996).
PCR amplification using the real-time PCR was performed as described above.
Gene disruptions were confirmed by PCR amplification using the isolated genomic DNAs as templates.
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