Torres, J., Asada, H. H., "High-Gain, High Transmissibility PZT Displacement Amplification Using a Rolling-Contact Buckling Mechanism and Preload Compensation Springs," Transactions on Robotics, Vol. 30, Issue 4, pp. 781-791, August 2014.
PCR amplification using a pair of primers designed from these sequences successfully identified the clones containing these high similar microsatellite sequences before sequencing.
They were subjected to PCR amplification using a StepOnePlus Real-Time PCR System thermocycler (Thermo Fisher Scientific) under the following conditions: initial denaturation at 95 °C for 60 s; amplification: denaturation at 95 °C for 20 s, annealing at 55 °C for 30 s, elongation at 72 °C for 60 s, 40 cycles; final elongation at 72 °C for 5 min.
The integrated human TSHα promoter region could be distinguished from the endogenous rat TSHα promoter due to specific amplification using a downstream luciferase cDNA primer in ChIP assays.
Briefly, total genomic DNA was digested with a restriction enzyme (Nsp1), ligated to an appropriate adapter for the enzyme, and subjected to PCR amplification using a single primer.
The resulting first strand cDNA products were poly G-tailed followed by 30 rounds of PCR amplification using a single primer.
Flow cytometry to detect cells with diminished fluorescence was performed under FL-1 logarithmic amplification using a FACSCanto Flow Cytometer (BD Bioscience).
Prior to using this approach, we attempted RNA isolation and amplification using a commercially available kit, but the amplified RNA degraded after a few freeze-thaws.
Therefore, we performed quasi-random RT-PCR amplification using a whole transcriptome amplification (WTA) kit, according to the manufacturer's protocol with modifications, i.e. 70 cycles of PCR [21].
Using an Illumina Cluster Station, the diluted libraries were hybridized to an 8-lane flow cell, followed by cluster generation by isothermal amplification using a DGE-Small RNA Cluster Generation Kit v1.0 (Illumina, San Diego, CA).
The presence of a given element at a particular location in the genome was revealed by a PCR amplification using a primer complementary to the internal region of the TE and a primer designed in the flanking region.
