Sentence examples for amplification undertaken from inspiring English sources

By contrast, the HD2 library was reformatted to allow additional duplicated controls as part of the library amplification undertaken at the SRSF (Additional file 1A) - changes that support independent statistical estimates of the required number of controls per plate [ 20].

PCR amplification was undertaken using Pfu Turbo polymerase (Stratagene) and the PC2 primer.

Due to the difficult nature of the Sox10 sequence (GC content 55 74% for most exons), PCR amplification was undertaken using several different taq polymerases and cycling annealing temperatures (Table S3) and primers from the DOGSET of UC Davis (http://www.vgl.ucdavis.edu/dogset/) with one additional primer designed using Primer3.0 http://frodo.wi.mit.edu/primer3/.edu/primer3/

Classical RT PCR amplification was undertaken with the HotStartTaq DNA Polymerase (QIAGEN).

Three rounds of amplification were undertaken, and at each stage, the baculovirus titer was measured by plaque assay.

PCR amplification was undertaken for plain SYBR Green I detection in using Light Cycler system (Roche Diagnostics GmbH, Mannheim, Germany).

PCR amplification was undertaken for Sybr qPCR Mix (Takara) in Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems Japan, Tokyo, Japan).

Real time PCR was carried out on the iCycler iQ real time detection system and amplification was undertaken by using SYBR Green I detection.

Real-time PCR was performed on the iCycler iQ real time detection system and amplification was undertaken by using SYBER Green I detection.

PCR amplification was undertaken as described above but with increased annealing temperature to 65°C and the amplicons visualized on a 3% agarose gel.

After the initial denaturation step (94°C for 5 min), reverse transcription (RT -PCR amplification was undeRT -PCR consisting of 30 cycles of 94°C for 12 s, 60°C for 8 s, amplification8 s.