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TOP2A protein overexpression was investigated in 40 pairs of tumor and matched nontumor tissues; TOP2A gene amplification was investigated in 22 pairs of the tissues which also used for HER2 gene amplification study.
In the amplification study, we used a ribosomal DNA probe/primer set (Applied Biosystems) to identify either the total absence of amplification or inhibition of the PCR reaction caused by the quality of the extracted DNA.
Microsatellite analysis was carried out using 14 microsatellites loci as described in [ 19] and five polymorphic microsatellites loci found in a cross-species amplification study of T. tambroides using primers from other cyprinids [ 20]: Cyprinus carpio (MFW7 [ 21]), Barbus barbus (Barb37, Barb59, and Barb62 [ 22]), and Barbonymus gonionotus (Bgon13 [ 11]).
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This paper offers a general fuzzy random formulation, applicable to nonlinear soil amplification studies and hence to evaluations of local seismic hazard.
Further work should include the influence of deep sedimentary layers for future site amplification studies covering a broad frequency range.
Multiple gene amplification studies for the diagnosis of tubercular uveitis have not been reported, although a few studies have evaluated multiple targets as separate uniplex PCRs in other extrapulmonary conditions.
Finn et al. [7] examined the applicability of 1-D and 2-D site response analyses for amplification studies in Fraser Delta, British Columbia using recorded ground motions from 1996 Duvall earthquake.
Viral genome sequencing and targeted amplification studies have led to the important discovery that phage encode environmentally relevant genes of host origin [27], [29], [30], [32], [44], [57].
PCR amplification studies were also done on paraffin embedded tissue from the samples at diagnosis.
The biopsies were subsequently sent for lymphocytic identification and PCR amplification studies for T cell rearrangement on fresh tissue.
MDA has been the method of choice for a wider range of genome amplification studies, because it produces longer DNA products with extensive genome coverage.
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