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The amplification sequence consists of a sequence of π-pulses that repetitively interrupt MAS averaging of the CSA interaction.
The amplification sequence involved an initial 10-min cycle at 95 °C, followed by 40 cycles, each composed of a denaturation step (95 °C for 15 s) and a primer annealing/extension step (60 °C for 45 s).
Yield compared to the same amplification sequence performed using a bench-top thermal cycler decreased nonlinearly from 73% (at 1 mm/s) to 13% (at 3 mm/s) with shorter residence time at the optimal temperatures for the reactions due to increased flow rate primarily responsible.
CXCL12 coding gene sequences were retrieved from GenBank to determine the full amplification sequence.
Because single-cell sequencing relies on gene amplification, sequence bias and adequate genome coverage remain major challenges.
Initial amplification, sequence analysis and alignment for validation of putative SNPs between Williams 82 and PI 468916 were performed as described by Choi et al. [ 3].
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But NGS also has an unacceptably high inherent error rate caused by mistakes in amplification, sequencing, and detection.
In conclusion, 5′ UTR amplification sequencing and subsequent VP1 specific primer amplification ensures a high detection rate and good genotyping accuracy in the examination of clinical samples.
DNA preparation, amplification, sequencing, and general primers follow Erixon and Oxelman [18].
To expand our knowledge of this, nearly full genome amplification, sequencing and analysis was conducted.
The methods for amplification, sequencing and data analysis of DLA-DRB1, -DQA1 and -DQB1 were performed as previously described [21].
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