Sentence examples for amplification procedures from inspiring English sources

These assays are labor-intensive and may be prone to exogenous DNA contamination due to their use of nested PCR amplification procedures and lack of contamination control measures.

Absence of post amplification procedures allows rapid analysis with a greater sample throughput, yet with less risk of amplicon carry-over as reaction tubes are not opened.

After fluorescently identifying MCF-7 and HEK-293T cells, we sequenced their short tandem repeat (STR) domain to further confirm the identification results and to tell if cross-contaminations happened in cell lysing and gene amplification procedures.

The 2 sets of primers designed for the specific PCR amplification of A. ovis and A. marginale allow easy-to-detect and discriminate between the 2 pathogens, thus avoiding the time-consuming sequencing or multi-gene amplification procedures.

For that purpose, we have characterized introns 2 and 3 from most DQA1 alleles to design amplification procedures that were able to obtain complete exon 2 and 3 sequences from DQA1 genes.

Exogenous DNA can also be introduced during extraction or amplification procedures [35].

We compared the same RNA sample at different dilutions, using the "low starting material" amplification procedures to obtain labelled cRNA for the low concentrations (see Material and methods).

Except for an annealing temperature of 55°C and elongation time of 60 sec the amplification procedures were the same as described above.

The primer selection process and amplification procedures for RPM-TEI are designed to minimize the impact of primer integrity, primer stability, and sample stability on detection capability but these can never be completely alleviated.

Although whole-genome amplification by phi29 polymerase may add some bias and increase background noise, it is regarded the most unbiased approach compared to PCR-based DNA amplification procedures [28].

The latter methodology includes a high number of RT-PCR protocols, but for reasons of quality assurance in quality management systems the need for standardized nucleic acid amplification procedures with quality marks like the CE mark increases more and more.