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In the second part, we illustrate the different applications of a specific statistical modeling technique, the bootstrap protocol, on either the amplification of data, the elimination of correlation existing between measured parameters or, out of a set of parameters, identification of the most relevant parameter for further statistical analysis.
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Validation of such data is important prior to their downward continuation because of amplification of the data errors through this process.
We tested functionalities of the front-end circuit; demultiplexing and amplification of the data signal.
Amplification of missing data (favors single SNPs).
The quality of MIN-6 and αTC1.6 cDNA was confirmed by PCR amplification of cyclophilin (data not shown).
Sequencing of the DNA fragments confirmed amplification of LCN7 cDNA (data not shown).
Plasmid integration into MiaPaCa2 cells was confirmed by PCR amplification of genomic DNA (data not shown).
Gene-specific primers for these selected genes resulted in comparable amplification efficiency as shown by amplification of genomic DNA (data not shown).
These primers were then used for subsequent PCR amplification of the larger data set.
Fluorescence was measured continuously to confirm amplification of specific transcripts (data not shown).
DNA quality of samples was confirmed by amplification of spindlin DNA (data not shown; Itoh et al. 2001).
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CEO of Professional Science Editing for Scientists @ prosciediting.com