The process and amplification materials used can be replicated in other districts in Uganda and Africa to enhance patient safety.
Consequently, trinucleotide threading represents a viable alternative for parallel STR amplification, producing material well suited for gel identification.
Whole genome amplification provided material for at least six of these libraries.
Primers were designed surrounding the morpholino binding site for PCR amplification (supplementary material Table S3).
Overexpression of MYB in cell culture was sufficient to turn on FOXJ1 expression, although it did not on its own induce centriole amplification (supplementary material Fig. S7).
In addition, similar to MMC, another ICL inducer, cis-platin, also induced robust centrosome amplification (supplementary material Fig. S7), suggesting that centrosome amplification is likely an integral part of the ICL induced response.
Microarray data were derived from custom microarray patterns, with probes placed in every exon and exon-exon junction, and a random-priming amplification protocol (Materials and Methods).
We hypothesized that primers with a GC-rich tail could be multiplexed and linearly amplified to avoid primer interactions without introducing allelic bias during amplification (see Materials and Methods for Multiplexed Linear Amplification).
The PCR method we employed for Hsp83 gene-specific small fragment amplification (see Materials and Methods) is proven to be highly sensitive in detecting any insertion/deletion mutation.
Ten target loci (Table 1) were selected based on three criteria: amenability to L1 insertion, lack of L1 sequences, and suitability for efficient long PCR amplification (see Materials and Methods).
Referral bias will typically be known to readers based upon the institution or institutions from which the study arises, although occasionally referral bias requires amplification in Materials and Methods.
