Sentence examples for amplification marker from inspiring English sources

For the stable production of cMAbs, we constructed 4 major different vector systems with a dihydrofolate reductase (DHFR) amplification marker, and optimized transfection/selection conditions in mammalian host cells with the single-gene and/or double-gene expression system.

To establish a series of technology for production of stable cMAbs and to evaluate which type of expression vector gives us a maximum performance for cMAb production, four major different expression systems with a DHFR amplification marker were generated by re-engineering the pIRES-DHFR vector.

The amplification marker leu2d (black arrow in Fig. 4A) has slight transcriptional activity and can complement leucine auxotrophy only if amplified.

CHO contigs aligned to DHFR, the amplification marker, and placental alkaline phosphatase, the recombinant protein produced in this cell line, were chosen because of the interest in these genes for cell line development efforts.

The bin distance was similar to that generated by RAD-Seq (0.69 cM) [ 5], but was shorter than that maps constructed using randomly selective amplification markers mainly (1.86 cM) [ 24] or the specific length amplified fragment sequencing (SLAF-seq) technology (1.20 cM) [ 4].

After removing patients with particularly favorable (CTNNB1 mutation, extensive nodularity) or unfavorable (MYC amplification) markers, a risk score for the remaining "intermediate molecular risk" population dependent on age, M-stage, pattern of synaptophysin expression, and MYCN copy-number status was identified, with speckled synaptophysin expression indicating worse outcome.

The phenotype of the 40 CHR markers was dominant, and primer pairs for CHR markers directed PCR amplification of marker sequence from Col-0 DNA and not from Ty-0 DNA.

SSR markers that were not polymorphic were used in the target region amplification polymorphism marker system (TRAP).

(2) No prior subcloning of BAC clone fragments or PCR amplification of marker cassettes flanked by homology regions is needed.

Moreover, results of clone library analyses are always biased by the choice of primers that are used for amplification of marker gene fragments and cloning efficiencies.

Twenty-five individual plants were used for DNA extraction, PCR amplification, SSR marker validation and diversity analysis.