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For a medium-pressure operation and low-frequency excitation, the amplification index is low and constant.
Acoustic amplification/attenuation and their intensities are judged by the normalized amplification index derived from Rayleigh's criterion.
As frequency increases further, the amplification index increases appreciably because of an intense accumulation effect, and its peak value exceeds that found for low-pressure operation.
In the low-pressure regime, the amplification index remains low and constant at low frequencies, which indicates a relatively weak acoustic amplification.
As acoustic frequency increases, finite-rate chemistry is enhanced through a nonlinear accumulation of heat release rate, leading to a high amplification index.
Using fluorescence in situ hybridization (FISH), we observed that 5 of the 12 cell lines had an AURKA amplification index (AI) (percentage of cells with more than three signals) >60%.
Similar(51)
Prior to amplification, indices to allow for sample multiplexing were incorporated.
The amplification indices for most (84%, or 27/32) cases with gene amplification, ranged between 1.8- and three-fold, indicating that the locus gained up to two copies of c- myc in the majority of the cases.
As shown in Table 1 > -wrap-foot>, 32 oft of 46 (70%) cases were gene amplified for c- myc, whereas only 30% (14/46) of the cases showed amplification indices lower than the cut-off value.
The same method was used to estimate the association of the amplification indices with the c-Myc protein expression levels, and the association of the mRNA expression levels with the protein expression levels.
An aliquot of the cells was sent to Cellecta for DNA extraction, PCR amplification without index tags, and barcode quantitation by deep sequencing.
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