The phrase "amplification in the second" is correct and usable in written English.
It can be used in contexts discussing music, audio, or any situation where an increase in volume or intensity is being referenced, particularly in relation to a second element or instance.
Example: "The amplification in the second section of the piece really enhances the emotional impact."
Alternatives: "increase in the second" or "boost in the second".
If you're going to take the kind of heat the City Opera has for introducing amplification in the first place, you might as well do it when needed.
Fourth, the 100-fold dilution after the linear amplification in the first step decreases the genomic DNA concentration to avoid non-specific amplification of the LAD1 and LAD3 primers with genomic DNA.
Amplification was detected in re-occurrences from seven of these 23, none of whom showed amplification in the first tumour sample.
The Gateway® cloning system uses a nested PCR method involving two rounds of amplification in which the second round uses the product of the first round as template.
Differential amplification occurred in the second round using the equivalent of 0.2 µL of the first round reaction as input.
Public health reference laboratories in Victoria and WA developed pH1N1-specific nucleic acid amplification tests in the first week of May.
The amplification program in the first PCR cycle was 60 min at 43°C then 2 min 30s at 94°C followed by 40 cycles with settings 20 s at 94°C, 20 s at 50°C, and 60 s at 72°C.
Furthermore, any two step approach (even one which has a limited number of amplification cycles in the first step) increases the risk of cross-contamination between samples when performing the second step.
The consistency with which these ratios, or relative frequencies, are observed suggests that they are empirical probabilities of mismatch amplification (mispriming) in the first cycle or first few cycles.
Then prior to PCR amplification the uracil in the second strand is degraded using Uracil-N-Glycosylase (UNG).
