The striking amplification and diversification in the number of genes encoding beetle PCWDEs can potentially be related to the presence, in plants, of inhibitors of PCWDEs, which have been implicated in defence against phytopathogens, especially fungi [2], [29].
The great diversity of the B-lymphocyte repertoire implies that their interaction with the antigen induces a clonal expansion process (positive selection) resulting in the amplification of the number of clones and the production of specific antibodies against the pathogen or the vaccine in biologically relevant quantities.
Several studies have shown that starting with minute samples in the initial amplification round, the number of genes detected with sufficient signal for further analysis decreased [ 14- 16].
This capacity to predict held for all concentrations tested including amplification failures when the number of molecules in the sample fell below the probability of amplification (see Additional file 1).
A modified form of fluorescent PCR approach, which detects the end products by fluorometric scanning after amplification, instead of by real-time, dynamic monitoring during the amplification process, improved the number of targets detectable in a single tube to six [6].
As such, difficulties in determining amplification efficiency limit the number of targets and reaction conditions that can be tested.
Moreover, alignment parameters can be set to reduce non-specific amplification by increasing the number of mismatches in the identification of unique loci in the genome, and accuracy can be improved by decreasing the number of mismatches in the analysis of variation between populations.
To avoid read count amplification in cases where the number of tied high-scoring matches was >1, the nucleotide and phosphate scores from the query contig were appropriately normalized and distributed evenly across the new contigs.
Explosive amplification results in a rapid increase in the number of infected and infective vector mosquitoes.
The double round amplification data revealed a slight reduction in the number of "present" calls.
