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If you're going to take the kind of heat the City Opera has for introducing amplification in the first place, you might as well do it when needed.
Fourth, the 100-fold dilution after the linear amplification in the first step decreases the genomic DNA concentration to avoid non-specific amplification of the LAD1 and LAD3 primers with genomic DNA.
Amplification was detected in re-occurrences from seven of these 23, none of whom showed amplification in the first tumour sample.
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For a noisy observation, however, small eigenvalues ζ uv can lead to significant noise amplification in the second term of (4) so the inverse problem becomes ill-posed.
Public health reference laboratories in Victoria and WA developed pH1N1-specific nucleic acid amplification tests in the first week of May.
The amplification program in the first PCR cycle was 60 min at 43°C then 2 min 30s at 94°C followed by 40 cycles with settings 20 s at 94°C, 20 s at 50°C, and 60 s at 72°C.
Furthermore, any two step approach (even one which has a limited number of amplification cycles in the first step) increases the risk of cross-contamination between samples when performing the second step.
The consistency with which these ratios, or relative frequencies, are observed suggests that they are empirical probabilities of mismatch amplification (mispriming) in the first cycle or first few cycles.
In a subsequent study, we improved the detection level of HIV-1 DNA levels with real-time PCR by including a pre-amplification step in the first PCR, followed by quantification with a second PCR [ 8].
Differential amplification occurred in the second round using the equivalent of 0.2 µL of the first round reaction as input.
The purified amplicons from the first PCR were used as templates in a second PCR with the same amplification condition used in the first PCR with the exception of using 454 fusion-tailed primers in a 30-cycle amplification regime.
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