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Effect of non-extension dimers on amplification efficiency.
The amplification efficiency was equal for all genes.
The amplification efficiency is enhanced.
All primers were tested for their amplification efficiency according to standard methods.
Clones revealing low amplification efficiency of the PCR products compared to the control were candidate mutants.
Results: The amplification efficiency was found to be 102%.
Ruijter, J. M. et al. Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.
Ruijter, J. M. et al Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.
Amplification efficiency was calculated by dilution.
Amplification efficiency varied among the other fish species.
PCR amplification efficiency was established by means of calibration curves.
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