Sentence examples for amplification designed from inspiring English sources

The NR2A, NR2B, NR2C, NR2D, NR3A, and GAPDH mRNA transcripts were quantified using the oligonucleotide primers for real-time PCR amplification designed based on sequences published in the Harvard Primer Bank and the NCBI primer-designing tool where the sequences were blasted.

MR made the whole genome amplification, designed some markers, made and analyzed the genotyping.

Briefly, 500 ng of DNA isolated from the prostate were subjected to PCR amplification designed to detect polyomaviruses and HPV [ 4, 8, 11].

Exome sequencing results for prioritized variants were validated using specific primers for polymerase chain reaction amplification designed using Primer3Plus (http://primer3plus.com/cgi-bin/dev/primer3plus.cgi) and Sanger sequencing (GATC Biotech, Cologne, Germany).

It is however possible that, in addition to the deletion allele, a second functional copy of ceh-63 was present in the genome, remained after back-crossing and was modified so as to be missed in PCR amplification designed to detect undeleted versions of the gene.

The emergence of methods to study the transcriptome initially necessitated the production of relatively large quantities of RNA from experimental systems, and Eberwine and Van Gelder's T7-based system of In vitro Transcription5 6 became the first standard protocol for RNA amplification designed to fulfill these requirements.

Primers for all amplifications, designed with PerlPrimer v1.1.10 (freeware by Owen Marshall), were located on exon-exon borders to prevent amplification of potentially contaminating genomic DNA.

Primers for qPCR monitoring of phage amplification were designed by using Beacon Designer™ program (Premier Biosoft Int., http://www.premierbiosoft.com).

A hybrid amplification design consisting of distributed Raman amplification and EDFA (Raman/EDFA), which has led to significant enhancement in system performance, is also described.

This chapter presents a new strain amplification design, named a "nested rhombus" multilayer mechanism, that increases strain exponentially through its hierarchical cellular structure.

This study can provide a theoretic foundation for the amplification design of the moving-bed reactor in the poly-generation system.