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Raw amplification data were exported and further analysis and statistical tests were done using Microsoft Excel 2010.
Amplification data were collected and analyzed with the SmartCycler system software.
Bio-Rad iQ5 Optical System software (version 1.0, 2005) was used to analyze the amplification data.
Amplification data were collected with an Applied Biosystems ViiA7 detector and analyzed with ViiA7 v 1.2.4 software (Life Technologies).
The integration of the recombinant expression cassette into the yeast genome was confirmed by PCR using the same primers used for gene amplification (data not shown).
Deletion products were not observed during amplification (data not shown).
During the same assay, mouse Gapdh showed robust amplification (data not shown).
All samples were checked for one intronic SNP without finding any amplification (data not shown).
Amplification data were analyzed using Sequence Detection Version 1 software (Applied Biosystems).
To confirm the amplification data a dot blot reaction was performed.
Amplification data was analyzed using Rotor-Gene Version 6.0 (Build 27) (Corbett Research, Mortlake, NSW, AU).
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