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multiple annealing and looping based amplification cycles.
Amplification cycles were repeated for 40 times.
c Multiple annealing and looping based amplification cycles (MALBAC).
The amplification cycles were 25 and 35 for leaf and root sample; respectively.
Several such amplification cycles occur.
For the 2,000 cells, 17 amplification cycles were used.
Initially, all samples were subjected to 27 amplification cycles.
For each PCR run, the samples underwent 40 amplification cycles.
The threshold cycle value of the individual measurements did not exceed 0.5 amplification cycles.
The temperatures and amplification cycles were the same used for the test patient DNA.
The number of amplification cycles was maintained low (10 cycles) to prevent a potential PCR bias.
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