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Furthermore, the proposed strategy was simple and sensitive, avoiding the adding of any coreactant into working solution for signal amplification compared with traditional ECL-based assay.
However, high levels of G-protein enrichment and receptor localization in lipid rafts provide a high density of reactants which can result in signal amplification compared with G-protein signaling without lipid rafts.
The overall survival was only slightly lower for patients with KIT amplification compared with patients who had a KIT mutation.
Our study also demonstrates the advantage of specific target gene amplification compared with 16S rDNA gene amplification.
From the results, we can see that most of the edge products have been successfully amplified except for that of rpsN, which displayed a relatively weak amplification compared with the others.
Thus, while cancer cells in culture exhibit much higher levels of mitotic activity and lower levels of centrosome amplification compared with cancer cells within patients' tumors, cancer cells in culture and in tumors display the common features of centrosome clustering in interphase as well as in mitosis.
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To assess the prognostic impact of RTK amplifications in gastric cancer, we performed a survival analysis comparing the clinical outcome of patients bearing tumours with RTK amplifications compared with patients with tumours lacking RTK amplification.
Large DCIS cases were found to have fewer copy number gains of MYC (40.9 % of large and 66.6 % of small DCIS) and of ZNF217 (27.2 % of large and 47.6 % of small), and in cases with an above-median Ki-67 index, large DCIS lesions showed fewer amplifications compared with small lesions [ 68].
Among the FISH-positive samples, EGFR gene mutation occurred more frequently in samples with gene amplification compared to those with high polysomy, suggesting that EGFR mutation and gene amplification should be used as clinical decision parameters to predict response to EGFR-targeting therapy.
However, there was no statistically significant difference in survival for patients with cancers having ESR1 amplification compared to patients with non- ESR1 amplified cancers containing the highest level of ERα protein (P = 0.09).
Considering that MeDIP approach employed Klenow for amplification of purified methylated DNA fragment, it generally has lower amplification efficiency compared with the PCR based amplification employed in DMH and MMASS.
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