The phrase "amplification by using" is correct and usable in written English.
It can be used in contexts where you are discussing the process of enhancing or increasing something through a specific method or tool.
Example: "The researcher focused on amplification by using advanced algorithms to improve data accuracy."
Alternatives: "enhancement through" or "increase by employing".
High-sensitivity detection relies upon quantification of DNA amplification by using polymerase chain reaction (PCR), while the simplified, low-sensitivity detection can be obtained through measurement of oxygen consumption due to respiration; importantly, both techniques utilize the same type of electrode.
Second strand synthesis was performed by using E. coli DNA polymerase I and the double-stranded cDNA was used for in vitro transcription (IVT) for cRNA amplification by using Megascript T7 kit (Ambion).
Total RNA was reverse-transcribed by using the First Strand cDNA Synthesis Kit (AMV, Roche) in a total volume of 20 µl. cDNA was subjected to PCR amplification by using the Expand High Fidelity PCR System (Roche Diagnostics).
The same amount of template was subjected to 25 cycles of PCR amplification by using β-actin primers as an internal standard so that quantitation of mRNA levels could be corrected among the samples.
In cell extracts, the relative amount (AR(E)) of each RNA was first calculated from the threshold cycle (CT) of each cDNA amplification by using the following equation: AR(E) = 2−ΔCT = 2− CT of test RNA – CT of reference RNA).
The relative amount (AR IP)) of each hY RNA and 5S rRNA in immunoprecipitates compared with mock immunoprecipitates was calculated from the threshold cycle (CT) of each cDNA amplification by using the following equation: AR IP) = 2 CT of mock immunoprecipitate – CT of specific immunoprecipitate).
We removed these amplifications by using CTL epitope predictors (removing 2). on a per-sequence basis (removing 1)., and by only plotting their C-terminal position (remove 3. and 4).
In the case of DNA, the third aliquot was used to produce an amplified DNA backup by whole genome amplification (WGA) by using Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Little Chalfont, UK).
After amplification, we tested for the presence of single amplification products by using a melting step using 0.5°C increments between 55° and 99°C and 1-sec hold reads.
By contrast, amplification performed by using primers xylHs-F and xylHs-R produced a single PCR fragment, of the expected length, when DNA from S. xylosus, S. haemolyticus, S. intermedius and S. kloosii were used as template.
We then modeled these site amplification factors by using analog filters (Eq. 8).
