The phrase "amplification buffer" is correct and usable in written English.
It can be used in contexts related to electronics, audio engineering, or signal processing where a buffer is used to amplify a signal.
Example: "The technician adjusted the amplification buffer to enhance the audio quality during the live performance."
Alternatives: "signal booster" or "gain buffer".
The reaction mixture (50μl) consisted of 1xPCR MIX: PCR amplification buffer, 0.05 U/μl Taq DNA polymerase, 0.4mM of each dNTP, 4mM MgCl2 (FERMENTAS), forward and reverse primers at 0.1mM and 3.5 μl of template DNA.
Following six PBST washes the samples were incubated for 30 minutes in the TSA-Fluorecein at a 1∶100 dilution in the TSA Amplification Buffer (0.004% H2O2 in 0.1 M borate buffer, pH 8).
For tyramide staining for endogenous Teashirt in H4 neuorglioma and neuron cultures, either AlexaFluor 488 or AlexaFluor594 dyes (Invitrogen, Carlsbad, CA) were diluted in 1∶100 in amplification buffer with 0.015% H2O2 and incubated for 5 10 minutes.
Amplifications contained: 0.200 nM forward and reverse primer, 200 µM dNTPs, 1 mg/ml bovine serum albumin, 1 x amplification buffer with 2 mM MgSO4, 0.025 U/µl reaction volume Taq DNA Polymerase (New England Biolabs, Ipswich, MA) and 10 ng of template DNA brought to a total volume of 15 µls with nuclease-free H20.
All the PCR reactions were carried out with pfx DNA polymerase (Invitrogen) with the amplification buffer used at 2X and the enhancer solution used at 3X, and with the following program: initial denaturation for 5 minutes at 94°C; 32 cycles of 94°C for 15 seconds, 55°C for 30 seconds, 68°C for 1 minute; and followed by 68°C for 7 minutes.
diluted at 1 50 in amplification buffer for 10 minutes.
In addition, PCR amplification buffers and enzymes have been decontaminated by treatment with heat-sensitive DNA nucleases [ 16].
Each dried pellet was resuspended in 250 μL of a solution containing 12.5 mg glass beads (Glass Controlled Pore, 120 200 μm mesh; Sigma), PCR amplification-buffer, and Proteinase K (0.6 mg/mL).
The RT PCR was performed in 25 μl mixture containing 20 μl of amplification mix (buffer, dNTPs, Taq gold polymerase, Rox passive fluorocrome, primers and MGB Eclipse probe) and 5 μl of purified DNA.
On the following day, species-specific HRP secondary solution (Vector Laboratories Inc., Peterborough, UK) was applied for 30 minutes, before fluorescein-labelled antibody in tyramide signal amplification (TSA) buffer (1 500, Perkin Elmer, Massachusetts, UK) was applied for eight minutes.
By making the source of information, in this case the irregular migrants, difficult to reach through the amplification of buffers, all other agencies and bodies are discredited by the government, as they cannot access the source and therefore are represented as not fully 'understanding' the issue at hand.
